Journal: medRxiv

Article Title: Biofabrication of multiplexed electrochemical immunosensors for simultaneous detection of clinical biomarkers in complex fluids

doi: 10.1101/2022.03.18.22272576

Figure Lengend Snippet: Specificity and cross-reactivity test for different biomarkers of Myocardial Infarction (MI) and Traumatic Brain Injury (TBI) done in 96 well plate. a) Specificity and cross-reactivity of NT-proBNP antigen against different non-specific capture and detection antibodies (anti-cTnITC, anti-BNP, anti-GFAP, and anti-S-100b) along with specific detection with anti-NT-proBNP capture and detection antibody at different concentrations of NT-proBNP. b) Specificity and cross-reactivity of BNP antigen against different non-specific capture and detection antibodies along with specific detection with anti-BNP capture and detection antibody at different concentrations of BNP. c) Specificity and cross-reactivity of GFAP antigen against different non-specific capture and detection antibodies along with specific detection with GFAP capture and detection antibody at different concentrations of GFAP. d) Specificity and cross-reactivity of S-100b antigen against different non-specific capture and detection antibodies along with specific detection with S-100b capture and detection antibody at different concentrations of GFAP.

Article Snippet: Three working electrodes were spotted with respective capture antibodies diluted in PBS [anti-BNP (HyTest, no. 50E1cc), anti-NT-proBNP (Medix Biochemica, no. 100 521), anti-cTnI (Abcam, no. ab243982), anti-cTnI-TC (Advanced ImmunoChemical, no. 2-TIC-rc) anti-GFAP (HyTest, no. GFAP83cc), anti-S100b (HyTest, no. 8B10cc)], anti NF-L (Uman Diagnostics, no. 27016), anti-IL-6 (Abcam, ab246838), anti-IL-8 (Abcam, ab215402), anti-IL-18 (Abcam, ab218185), anti-VEGF (R&D system DY493-05), and anti-Ag85-B (Abcam, ab36731).

Techniques:

Journal: medRxiv

Article Title: Biofabrication of multiplexed electrochemical immunosensors for simultaneous detection of clinical biomarkers in complex fluids

doi: 10.1101/2022.03.18.22272576

Figure Lengend Snippet: Specificity and Multiplexed detection for different biomarkers of MI and TBI using antifouling nanocomposite coated EC Biosensors. a) Calibration curve for multiplex detection of cTnITC using plasma sample on the EC Biosensor with four different capture antibodies on each electrode (anti-cTnITC, anti-S-100b, anti-GFAP, and anti-NT-proBNP). b) Calibration curve for multiplex detection of increasing concentration of cTnITC (left y-axis) and decreasing concentration of GFAP (right y-axis) using plasma sample (c) and whole blood (d) on the EC Biosensor with four different capture antibodies on each electrode. e) Calibration curve for multiplex detection of increasing concentration of s100 (left y-axis) and decreasing concentration of GFAP (right y-axis) using whole blood on the EC Biosensor with four different capture antibodies on each electrode. Error bars represent the s.d. of the mean; n = 3.

Article Snippet: Three working electrodes were spotted with respective capture antibodies diluted in PBS [anti-BNP (HyTest, no. 50E1cc), anti-NT-proBNP (Medix Biochemica, no. 100 521), anti-cTnI (Abcam, no. ab243982), anti-cTnI-TC (Advanced ImmunoChemical, no. 2-TIC-rc) anti-GFAP (HyTest, no. GFAP83cc), anti-S100b (HyTest, no. 8B10cc)], anti NF-L (Uman Diagnostics, no. 27016), anti-IL-6 (Abcam, ab246838), anti-IL-8 (Abcam, ab215402), anti-IL-18 (Abcam, ab218185), anti-VEGF (R&D system DY493-05), and anti-Ag85-B (Abcam, ab36731).

Techniques: Multiplex Assay, Concentration Assay

Journal: Talanta

Article Title: A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis.

doi: 10.1016/j.talanta.2022.123759

Figure Lengend Snippet: Fig. 1. Picture of the fabricated chip once passivated using epoxy resin and before to be used for quantify NT-proBNP in saliva samples.

Article Snippet: NTproBNP (Cat. No. 8NT2, declared concentration 1.2 mg/mL) and antiNT-proBNP antibody (Cat. No. 4NT1cc 15F11 cc, declared concentration 6.7 mg/mL) were purchased from HyTest (Finland).

Techniques:

Journal: Talanta

Article Title: A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis.

doi: 10.1016/j.talanta.2022.123759

Figure Lengend Snippet: Fig. 2. Example of Nyquist plots obtained by analysing NT-proBNP calibration samples in PBS (0.02, 0.05, 0.1, 0.3, 0.5, and 1.0 pg/mL). Reference plot (black, baseline) is RAb (R2 due to the presence of anti-NT-proBNP mAb only immo bilised onto the sensing surface). EIS frequency was ranged from 10 Hz to 10 KHz, with Eac 75 mV and Edc 0 V. The equivalent circuit model [R1 + Q2/R2] used for EIS data fitting is on the lower right.

Article Snippet: NTproBNP (Cat. No. 8NT2, declared concentration 1.2 mg/mL) and antiNT-proBNP antibody (Cat. No. 4NT1cc 15F11 cc, declared concentration 6.7 mg/mL) were purchased from HyTest (Finland).

Techniques:

Journal: Talanta

Article Title: A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis.

doi: 10.1016/j.talanta.2022.123759

Figure Lengend Snippet: Fig. 3. Results from interference study obtained by analysing STDs containing NT-proBNP and other HF biomarkers (e.g. TNF-α, IL-10, and cortisol) in the concentration range 5–20 pg/mL using the IMFET functionalized with anti-NT- proBNP mAb.

Article Snippet: NTproBNP (Cat. No. 8NT2, declared concentration 1.2 mg/mL) and antiNT-proBNP antibody (Cat. No. 4NT1cc 15F11 cc, declared concentration 6.7 mg/mL) were purchased from HyTest (Finland).

Techniques: Concentration Assay

Journal: Talanta

Article Title: A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis.

doi: 10.1016/j.talanta.2022.123759

Figure Lengend Snippet: Fig. 4. SAM curves obtained from analysis of (A) SAM sample 1, (B) SAM sample 2, and (C) SAM sample 3. Each sample was prepared by spiking a PSS aliquot with 1 pg/mL of NT-proBNP. The red-edged boxes highlight the interception of each curve on x-axis. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: NTproBNP (Cat. No. 8NT2, declared concentration 1.2 mg/mL) and antiNT-proBNP antibody (Cat. No. 4NT1cc 15F11 cc, declared concentration 6.7 mg/mL) were purchased from HyTest (Finland).

Techniques: